Application

Research CategoryInflammation & Immunology

Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)

Optimal working dilutions must be determined by the end user.

This Anti-Proteinase-Activated Receptor 2 Antibody, N-terminal, extracellular is validated for use in IH(P) for the detection of Proteinase-Activated Receptor 2.

Research Sub CategoryInflammation & Autoimmune Mechanisms

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Proteinase-activated receptor 2 (PAR2) is a member of the proteinase-activated receptor subfamily. It is activated through proteolytic exposure of an occult tethered ligand by trypsin and trypsin-like proteases. This is in contrast to other members of the subfamily which are activated by the protease thrombin. PAR2 has been implicated in acute inflammatory response, asthma, and pain transmission. PAR2 expression has been documented in the periphery. ESTs have been isolated from adrenal, brain, breast, heart/melanocyte/uterus, kidney, lung, and vessel libraries.

Immunogen

Synthetic peptide corresponding to a sequence within the N-terminal extracellular domain of human PAR2. The immunogen has 70% homology with mouse and 60% homology with rat.

Epitope: N-terminal, extracellular

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Peptide-affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide

Specificity

Recognizes human PAR2. The specificity of this antibody has been confirmed by IHC staining of human pancreas.

Storage and Stability

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.